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The architecture of responsive polymeric ligands on protein binding and recovery

Author:
Liu, Zizhao, Wickramasinghe, S. Ranil, Qian, Xianghong
Source:
RSC advances 2017 v.7 no.44 pp. 27823-27832
ISSN:
2046-2069
Subject:
adsorption, binding capacity, bovine serum albumin, composite polymers, hydrophilicity, hydrophobic bonding, ligands, polymerization, sorption isotherms
Abstract:
Ligand design and optimization are critical for protein purification during downstream processing. Here the effects of three dimensional architecture of salt- and thermo-responsive polymeric ligands on binding and recovery of bovine serum albumin (BSA) were investigated. The comb-like salt-responsive copolymers consisting of hydrophilic backbone (poly(hydroxylethyl methacrylate) (poly(HEMA))) and branched responsive (poly(N-vinylcaprolactam) (PVCL)) chains have been successfully grafted on membrane substrates as hydrophobic interaction ligands for protein capture and recovery. Protein binding capacity, binding kinetics and recovery were systematically investigated as a function of backbone chain density and chain length. Atom-transfer radical polymerization (ATRP) was used to control the polymer chain length and chain density of grafted brushes. Our results show that the architecture of these polymeric ligands has a significant impact on protein binding and recovery. The protein binding isotherm was found to follow the Freundlich model suggesting a multi-layer adsorption mechanism.
Agid:
6415802