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Matrix-Assisted Laser Desorption Ionization Imaging and Laser Ablation Sampling for Analysis of Fungicide Distribution in Apples

Author:
Pereira, Igor, Banstola, Bijay, Wang, Kelin, Donnarumma, Fabrizio, Vaz, Boniek G., Murray, Kermit K.
Source:
Analytical chemistry 2019 v.91 no.9 pp. 6051-6056
ISSN:
1520-6882
Subject:
apple peels, apples, desorption, electrospray ionization mass spectrometry, fruits, image analysis, imazalil, ionization, quantitative analysis
Abstract:
A combination of matrix-assisted laser desorption ionization (MALDI) imaging and infrared (IR) laser ablation sampling with offline electrospray ionization mass spectrometry (ESI-MS) was used to determine the distribution of the fungicide imazalil in apples. MALDI images were used to determine the penetration depth of imazalil up to 7 days after its application. IR laser ablation sampling and ESI-MS were used to quantify the rate of penetration of the fungicide, which was determined to be approximately 1 mm per day. Imazalil concentration decreased in the apple skin over the course of the experiment, and after 7 days the fungicide was detected at 0.015 ppm 6 mm inside the apple. Approximately 60% of the pesticide remained in the skin after 7 days. This work demonstrates the utility of MALDI imaging for spatial localization of fungicide in fruit in combination with IR laser ablation and ESI-MS for quantitative analysis.
Agid:
6422916