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Photocontrollable Proteins for Optoacoustic Imaging
- Mishra, Kanuj, Fuenzalida-Werner, Juan Pablo, Ntziachristos, Vasilis, Stiel, Andre C.
- Analytical chemistry 2019 v.91 no.9 pp. 5470-5477
- blood, hemoglobin, image analysis, light microscopy, lipids, ultrasonics
- Photocontrollable proteins revolutionized life-science imaging due to their contribution to subdiffraction-resolution optical microscopy. They might have yet another lasting impact on photo- or optoacoustic imaging (OA). OA combines optical contrast with ultrasound detection enabling high-resolution real-time in vivo imaging well-beyond the typical penetration depth of optical methods. While OA already showed numerous applications relying on endogenous contrast from blood hemoglobin or lipids, its application in the life-science was limited by a lack of labels overcoming the strong signal from the aforementioned endogenous absorbers. Here, a number of recent studies showed that photocontrollable proteins provide the means to overcome this barrier eventually enabling OA to image small cell numbers in a complete organism in vivo. In this Feature article, we introduce the key photocontrollable proteins, explain the basic concepts, and highlight achievements that have been already made.