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Flavobacterium aquariorum sp. nov., isolated from freshwater of the North Han River

Joung, Yochan, Jang, Hye-Jin, Song, Jaeho, Cho, Jang-Cheon
The journal of microbiology 2019 v.57 no.5 pp. 343-349
Flavobacterium pectinovorum, Flavobacterium saccharophilum, fatty acids, freshwater, lakes, nucleotide sequences, phosphatidylethanolamines, phylogeny, polyamines, quinones, ribosomal RNA, rivers, sequence homology, Korean Peninsula
A non-motile, yellow-pigmented bacterial strain, designated IMCC34762ᵀ, was isolated from a freshwater sample collected from Lake Cheongpyeong in Korea. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain IMCC-34762ᵀ formed a lineage within the genus Flavobacterium and was most closely related to F. pectinovorum DSM 6368ᵀ (98.3% sequence similarity), followed by F. piscis CCUG 60099ᵀ (98.3%), F. branchiicola 59B-3-09ᵀ (98.2%), and F. saccharophilum DSM 1811ᵀ (98.2%). The average nucleotide identity and the genome-to-genome distance between strain IMCC34762ᵀ and the closely related strains were 61–62% and 26–27%, respectively, indicating that IMCC34762ᵀ is a novel species of the genus Flavobacterium. The major fatty acids (> 5%) of strain IMCC34762ᵀ were summed feature 3 (C₁₆:₁ω6c and/or C₁₆:₁ω7c, 17.3%), iso-C₁₅:₀ (15.0%), iso-C₁₅:₀ G (9.0%), C₁₅:₀ω6c (7.4%), iso-C₁₅:₀ (7.4%), and iso-C₁₆:₀ (5.3%). The major respiratory quinone and polyamine were MK-6 and sym-homospermidine, respectively. The major polar lipids were phosphatidylethanolamine, an unidentified aminophospholipid, and an unidentified lipid. The DNA G + C content of strain IMCC34762ᵀ was 34.4 mol%. Based on the taxonomic data presented in this study, strain IMCC-34762ᵀ represents a novel species within the genus Flavobacterium, for which the name Flavobacterium aquariorum, sp. nov. is proposed. The type strain is IMCC34762ᵀ (= KACC 19725ᵀ = NBRC 113425ᵀ).