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Edaphovirga cremea gen. nov., sp. nov., isolated from the rhizospheric soil of Codonopsis clematidea

Xue, Jin-Yan, Zhang, Meng-Yue, Zhang, Yu, Cheng, Juan, Liu, Li-Cheng, Wu, Ying-Ying, Zhang, Tian-Yuan, Zhang, Yi-Xuan
The journal of microbiology 2019 v.57 no.5 pp. 337-342
Codonopsis clematidea, DNA, Rahnella, bacteria, catalase, chemotaxonomy, coagulation, fatty acids, hydrogen sulfide, isoprenoids, milk, new genus, nitrate reduction, nucleotide sequences, pH, phosphatidylethanolamines, phylogeny, quinones, ribosomal RNA, sodium chloride, soil, urease, China
A Gram-negative, facultatively anaerobic, non-motile, nonspore- forming, coccoid or rod-shaped and creamy-pigmented bacterium, designated SYP-B2100ᵀ, was isolated from the rhizospheric soil of Codonopsis clematidea in the Xinjiang Uygur Autonomous Region, China. The optimal growth occurred at 28°C, pH 5.0, in the absence of NaCl. The cells tested positive in catalase and methyl red tests but negative in oxidase, urease, gelatinase, milk coagulation, and peptonisation, H₂S production, nitrate reduction, and Voges-Proskauer tests. The major isoprenoid quinone was ubiquinone-8 (Q-8). The major cellular fatty acids were C₁₆:₀ and summed feature 8. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol. The 16S rRNA gene sequence of strain SYP-B2100ᵀ was the most similar to that of Rahnella inusitata DSM 30078ᵀ (96.9%) within the family Enterobacteriaceae. The genomic DNA G + C content of strain SYP-B2100T was 50.3 mol%. The combined data from the phylogenetic, morphological, physiological, biochemical, and chemotaxonomic analyses presented in this study support the conclusion that strain SYP-B2100T represents a novel species of a new genus, for which the name Edaphovirga cremea gen. nov., sp. nov. is proposed; the type strain is SYPB2100T (= CGMCC 1.5857ᵀ = DSM 105170ᵀ = KCTC 62024ᵀ).