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A bodipy based fluorescent probe for evaluating and identifying cancer, normal and apoptotic C6 cells on the basis of changes in intracellular viscosity
- Gupta, Neha, Reja, Shahi Imam, Bhalla, Vandana, Gupta, Muskan, Kaur, Gurcharan, Kumar, Manoj
- Journal of materials chemistry B 2016 v.4 no.11 pp. 1968-1977
- Western blotting, apoptosis, flow cytometry, fluorescence, image analysis, models, molecular rotors, monitoring, neoplasm cells, neoplasms, neuroglia, nuclear magnetic resonance spectroscopy, temperature, viscosity
- The applications of a bodipy based probe 1 for the identification of diseased cell population out of normal cells on the basis of changes in intracellular viscosity have been explored. Probe 1 works on the principle of restriction of rotation in viscous medium and the molecular rotor nature of probe 1 is supported by low temperature ¹H NMR and variable dihedral angle DFT and TD-DFT studies. More importantly, probe 1 is the first probe which shows its practical application in monitoring micro-viscosity changes in a cell based model system of undifferentiated, differentiated and apoptotic C6 glial cells. Further, probe 1 can effectively monitor the apoptosis pathway by showing an increase in fluorescence intensity from cancerous cells to apoptotic cells via real time live-cell video imaging. Moreover, the viscosity changes in living cells were proved by fluorescence lifetime imaging (FLIM) studies, flow cytometry using Annexin-V and Bcl-xl expression by immunocytofluorescence (ICC) and western blot analysis.