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A bodipy based fluorescent probe for evaluating and identifying cancer, normal and apoptotic C6 cells on the basis of changes in intracellular viscosity

Gupta, Neha, Reja, Shahi Imam, Bhalla, Vandana, Gupta, Muskan, Kaur, Gurcharan, Kumar, Manoj
Journal of materials chemistry B 2016 v.4 no.11 pp. 1968-1977
Western blotting, apoptosis, flow cytometry, fluorescence, image analysis, models, molecular rotors, monitoring, neoplasm cells, neoplasms, neuroglia, nuclear magnetic resonance spectroscopy, temperature, viscosity
The applications of a bodipy based probe 1 for the identification of diseased cell population out of normal cells on the basis of changes in intracellular viscosity have been explored. Probe 1 works on the principle of restriction of rotation in viscous medium and the molecular rotor nature of probe 1 is supported by low temperature ¹H NMR and variable dihedral angle DFT and TD-DFT studies. More importantly, probe 1 is the first probe which shows its practical application in monitoring micro-viscosity changes in a cell based model system of undifferentiated, differentiated and apoptotic C6 glial cells. Further, probe 1 can effectively monitor the apoptosis pathway by showing an increase in fluorescence intensity from cancerous cells to apoptotic cells via real time live-cell video imaging. Moreover, the viscosity changes in living cells were proved by fluorescence lifetime imaging (FLIM) studies, flow cytometry using Annexin-V and Bcl-xl expression by immunocytofluorescence (ICC) and western blot analysis.