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A fast-response two-photon fluorescent probe for the detection of Cys over GSH/Hcy with a large turn-on signal and its application in living tissues
- Wang, Jian-Yong, Liu, Zhan-Rong, Ren, Mingguang, Kong, Xiuqi, Lin, Weiying
- Journal of materials chemistry B 2016 v.5 no.1 pp. 134-138
- cysteine, cytotoxicity, edema, fluorescent dyes, image analysis, liver, skin lesions, tissues
- Cysteine (Cys), a small-molecule aminothiol, plays important roles in various physiological processes in connection with various diseases, such as skin lesions, edema, hair depigmentation and liver damage. We developed a novel two-photon fluorescent probe for sensing Cys in presence of GSH and Hcy in vivo. The two-photon fluorescent probe exhibited favorable properties, including fast response (about 20 min), good selectivity, and low cytotoxicity. Furthermore, it was successfully applied for imaging Cys in living cells and tissues.