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Histidine-enriched multifunctional peptide vectors with enhanced cellular uptake and endosomal escape for gene delivery

Author:
Meng, Zhao, Luan, Liang, Kang, Ziyao, Feng, Siliang, Meng, Qingbin, Liu, Keliang
Source:
Journal of materials chemistry B 2016 v.5 no.1 pp. 74-84
ISSN:
2050-7518
Subject:
DNA, biocompatibility, blood serum, circular dichroism spectroscopy, confocal microscopy, cysteine, cytotoxicity, flow cytometry, genes, histidine, light scattering, moieties, pH, particle size, peptides, proteolysis, transfection, trypsin, zeta potential
Abstract:
Peptide vectors offer a promising gene delivery approach because of their biocompatibility and ease of functionalization. This article describes the design and evaluation of a series of multifunctional peptides and their gene delivery abilities. The peptides were composed of a cell-penetrating segment, stearyl moiety, cationic amphiphilic α-helical segment, and cysteine and histidine residues. The proton sponge effect of histidine residues at low pH and the α-helical conformation should improve endosomal escape. Inclusion of d-type amino acids should improve proteolytic stability. The conformation, particle size and zeta potential of peptide/DNA complexes were characterized by circular dichroism and dynamic light scattering. Gene transfection efficiency was investigated by fluorescence-activated cell sorting and confocal microscopy. Transfection efficiencies of the designed peptide vectors were better than those of C₁₈-C(LLKK)₃C-TAT and Lipo2000. d-Type peptide C₁₈-c(llhh)₃c-tat showed three times higher transfection efficiency at N/P ratios of 6 and 8 than Lipo2000 in NIH-3T3 and 293T cells. All peptides showed lower cytotoxicity than Lipo2000 in NIH-3T3 and 293T cells. In the presence of trypsin or serum in vitro, d-type peptides showed better stability than l-type peptides. Overall, the designed histidine-enriched multifunctional peptide gene vectors promoted cellular uptake, endosomal escape and gene transfection.
Agid:
6424786