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Comparing mRNA expression levels to protein abundance in Magnolia denudata pollen following cryopreservation
- Xu, J., Liu, Y., Li, B. L., Liu, Q., Shi, Y., Peng, J. G., Jia, M. X.
- Acta horticulturae 2019 no.1234 pp. 113-118
- Magnolia denudata, cryopreservation, enoyl-(acyl-carrier-protein) reductase (NADH), gene expression, genes, messenger RNA, nitrogen, pollen, protein synthesis, proteins, quantitative polymerase chain reaction, transcription (genetics)
- Although genetic properties of a cell are determined by genes, genetic information is ultimately embodied in the function of the proteins. It has been known that there are four levels of regulation from gene to protein: transcriptional control, post-transcriptional control, translational control and post-translational control. In our previous study, eight differentially expressed proteins were identified from Magnolia denudata pollen cryopreservation. In order to further complete the molecular mechanism of cryopreservation, this study compared mRNA expression levels to protein abundance of enoyl-ACP reductase and putative dehydrogenase in M. denudata pollen at the pre-, during- and post- liquid nitrogen (LN) stages of cryopreservation, using quantitative real-time polymerase chain reaction. The results indicate that mRNA expression levels of two genes were similar following cryopreservation, with during- and post-LN showing almost equal expression level, but decreasing by half compared to pre-LN. These changes were both inconsistent with the protein abundance changes of these two genes, which suggest that the changes at protein abundance probably come from translational control or post-translational control, rather than transcriptional control or post-transcriptional control. It was revealed to some extent how the pollen cell responds to cryopreservation.