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Vitrification-based cryopreservation of Dendrobium cruentum Rchb. f. seeds

Prasongsom, S., Thammasiri, K., Narangajavana, J., Thiamadee, S., Cheunboonngarm, N., Panvisavas, N.
Acta horticulturae 2019 no.1234 pp. 157-166
Dendrobium, cryopreservation, epiphytes, exposure duration, extinction, germination, germplasm, nitrogen, plant tissues, seeds, sodium alginate, storage time, temperature, vitrification, water content, Thailand
Dendrobium cruentum Rchb. f. is one of the epiphytic orchids originated from southwestern Thailand. It faces extinction. The cultivation of this species was successful but there is a problem for long-term storage. Meanwhile, cryopreservation is a potential technique for long-term conservation of several biological germplasm based on plant tissue at ultra-low temperature of liquid nitrogen (LN, -196°C). The vitrification technique is the use of a plant vitrification solution especially PVS2 to provide a glassy state of plant materials. Under the ultra-low temperature, Tg, Tm and Tc were observed. From the experiment, encapsulation-vitrification and V cryo-plate methods were used by using 2% sodium alginate with various exposure times (0, 30, 60, 90 and 120 min) to determine the different water content. The maximum germination percentage (68.1%) was observed in V cryo-plate method with 60 min exposure time of PVS2 solution, while the encapsulation-vitrification method showed lower germination percentage (21.6%) under the same conditions.