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Vitrification-based cryopreservation of Dendrobium cruentum Rchb. f. seeds
- Prasongsom, S., Thammasiri, K., Narangajavana, J., Thiamadee, S., Cheunboonngarm, N., Panvisavas, N.
- Acta horticulturae 2019 no.1234 pp. 157-166
- Dendrobium, cryopreservation, epiphytes, exposure duration, extinction, germination, germplasm, nitrogen, plant tissues, seeds, sodium alginate, storage time, temperature, vitrification, water content, Thailand
- Dendrobium cruentum Rchb. f. is one of the epiphytic orchids originated from southwestern Thailand. It faces extinction. The cultivation of this species was successful but there is a problem for long-term storage. Meanwhile, cryopreservation is a potential technique for long-term conservation of several biological germplasm based on plant tissue at ultra-low temperature of liquid nitrogen (LN, -196°C). The vitrification technique is the use of a plant vitrification solution especially PVS2 to provide a glassy state of plant materials. Under the ultra-low temperature, Tg, Tm and Tc were observed. From the experiment, encapsulation-vitrification and V cryo-plate methods were used by using 2% sodium alginate with various exposure times (0, 30, 60, 90 and 120 min) to determine the different water content. The maximum germination percentage (68.1%) was observed in V cryo-plate method with 60 min exposure time of PVS2 solution, while the encapsulation-vitrification method showed lower germination percentage (21.6%) under the same conditions.