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Concise chemoenzymatic synthesis of heparan sulfate analogues as potent BACE-1 inhibitors

Author:
Li, Jun, Li, Jiarui, Sun, Tiantian, Cai, Chao, Shao, Meng, Yu, Guangli
Source:
Carbohydrate polymers 2019 v.217 pp. 232-239
ISSN:
0144-8617
Subject:
Pasteurella multocida, amyloid, binding proteins, drugs, enoxaparin, heparan sulfate, molecular weight, regioselectivity
Abstract:
Heparan sulfate (HS) and heparin, representative members of the glycosaminoglycans, possess distinct biological functions in terms of their specific interactions with hundreds of binding proteins. However, the structural properties of HS and heparin are complex due to their variable repeating motifs, different chain lengths and sulfation patterns. A concise chemoenzymatic approach has been developed to obtain well-defined low molecular weight (LMW) HS analogues. Pasteurella multocida heparosan synthase-2 (PmHS2) was utilized to fabricate the HS backbones with controllable chain lengths ranging from 14mer to 26mer. Moreover, regioselective and overall sulfation were conducted by chemical approach. The persulfated HS analogues exhibited more potent beta-site amyloid precursor protein (APP)-cleaving enzyme-1 (BACE-1) inhibitory activity than heparin and enoxaparin, and enhanced BACE-1 inhibitions were also found with the increasing molecular size of the HS analogues. This approach supplies the promising LMW HS analogues for the potential development of novel anti-Alzheimer's drugs.
Agid:
6429296