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Response of lipid biosynthesis in Chlorella pyrenoidosa to intracellular reactive oxygen species level under stress conditions

Zhang, Lei, Liao, Chunmei, Yang, Yingwu, Wang, Yong-Zhong, Ding, Ke, Huo, Danqun, Hou, Changjun
Bioresource technology 2019 v.287 pp. 121414
Chlorella pyrenoidosa, algae, biosynthesis, fatty acids, gene expression regulation, hydrogen peroxide, hydroxyl radicals, lipid content, superoxide anion, transcriptome
An increase in the total lipid content in algal cells under stress conditions is often accompanied by an increase in reactive oxygen species (ROS). However, the link between these two events is unclear. In this study, the regulatory mechanism of ROS formation on lipid accumulation in C. pyrenoidosa was investigated using a Fenton-like reaction. A high Spearman correlation coefficient of 0.901 was obtained between the Hydroxyl radical (OH) level and lipid content. Importantly, the increase in the total lipid content was clearly coupled with a significant increase in the intracellular OH concentration rather than increases in the H2O2 and O2− concentrations. Transcriptome data confirms that most of the differential expression genes (DEGs) involved in fatty acid and glycerolipid biosynthesis were up-regulated by the increased OH under stress conditions. These results reveal that lipid accumulation in algal cells was promoted by OH.