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Acquired protective immune response in a fish-myxozoan model encompasses specific antibodies and inflammation resolution

Author:
Picard-Sánchez, Amparo, Estensoro, Itziar, del Pozo, Raquel, Piazzon, M. Carla, Palenzuela, Oswaldo, Sitjà-Bobadilla, Ariadna
Source:
Fish & shellfish immunology 2019 v.90 pp. 349-362
ISSN:
1050-4648
Subject:
Sparus aurata, antibodies, blood serum, complement, cytotoxicity, enteritis, fish, gene expression, histopathology, immune response, immunoglobulin M, inflammation, interleukin-10, interleukin-1beta, intestinal mucosa, kidneys, models, parasites, peroxidases, phenotype
Abstract:
The myxozoan parasite Enteromyxum leei causes chronic enteritis in gilthead sea bream (GSB, Sparus aurata) leading to intestinal dysfunction. Two trials were performed in which GSB that had survived a previous infection with E. leei (SUR), and naïve GSB (NAI), were exposed to water effluent containing parasite stages. Humoral factors (total IgM and IgT, specific anti-E. leei IgM, total serum peroxidases), histopathology and gene expression were analysed. Results showed that SUR maintained high levels of specific anti-E. leei IgM (up to 16 months), expressed high levels of immunoglobulins at the intestinal mucosa, particularly the soluble forms, and were resistant to re-infection. Their acquired-type response was complemented by other immune effectors locally and systemically, like cell cytotoxicity (high granzyme A expression), complement activity (high c3 and fucolectin expression), and serum peroxidases. In contrast to NAI, SUR displayed a post-inflammatory phenotype in the intestine and head kidney, characteristic of inflammation resolution (low il1β, high il10 and low hsp90α expression).
Agid:
6430744