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Improving L-serine production in Escherichia coli via synthetic protein scaffold of SerB, SerC, and EamA

Author:
Tran, Kim-Ngan Thi, Eom, Gyeong Tae, Hong, Soon Ho
Source:
Biochemical engineering journal 2019 v.148 pp. 138-142
ISSN:
1369-703X
Subject:
Escherichia coli, carbon, enzymes, factories, glucose, markets, scaffolding proteins, serine
Abstract:
Although L-Serine has wide applications and great market potential, its application is limited by its high cost and low yields. Microbial factories have many advantages for producing L-serine from renewable carbon resources. To achieve L-serine production with high efficiency, three enzymes (SerB, SerC, and EamA) were physically re-localized by using a scaffold system GBD:SH3:PDZ. Such strategy was highly effective in improving the production of L-serine in Escherichia coli. The highest concentration of L-serine harvested was at 3.8 g/l after 48 h in strain E. coli with the scaffold GBD:SH3:PDZ at ratio of 1:2:1, reaching a high yield of 0.24 mol/mol glucose.
Agid:
6430845