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Co-fermentation of glucose and xylose from sugarcane bagasse into succinic acid by Yarrowia lipolytica

Ong, Khai Lun, Li, Chong, Li, Xiaotong, Zhang, Yu, Xu, Jingliang, Lin, Carol Sze Ki
Biochemical engineering journal 2019 v.148 pp. 108-115
Yarrowia lipolytica, bioreactors, biorefining, endo-1,4-beta-glucanase, enzymatic hydrolysis, feasibility studies, feedstocks, fermentation, glucose, hydrolysates, hydrolysis, pH, succinic acid, sugarcane, sugarcane bagasse, temperature, wastes, xylose
This study focused on the feasibility of Y. lipolytica PSA02004 co-utilising glucose and xylose from sugarcane bagasse hydrolysate in succinic acid (SA) fermentation. Optimum pH, temperature and cellulase dosage of enzymatic hydrolysis through optimisation were pH 5, 50 °C and 40 FPU/g, respectively. The hydrolysis yields for glucose and xylose were 0.35 ± 0.00 g/g and 0.15 ± 0.00 g/g, respectively. A feasibility study using a 2.5-L bioreactor demonstrated that mixed glucose and xylose could be utilised for SA production, with the resultant SA titre, yield and productivity of 28.2 ± 0.6 g/L, 0.55 ± 0.01 g/g and 0.36 ± 0.01 g/L/h, respectively. Then, the investigation of nutrient-limiting factor showed 40 g/L of initial glucose concentration and 8.4 g/L of initial xylose concentration containing hydrolysate and 2% CSL were the optimum medium composition in SA fermentation. SA production using sugarcane bagasse hydrolysate was conducted, in which SA titre, yield and productivity were 33.2 ± 0.3 g/L, 0.58 ± 0.01 g/g and 0.33 ± 0.01 g/L/h, respectively. The experimental results reported in this study show that glucose and xylose in sugarcane bagasse hydrolysate were successfully co-utilised by Y. lipolytica PSA02004 in SA production. This implies a sugarcane waste biorefinery could lead to a generic feedstock for the efficient production of succinic acid.