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Dysgonomonas massiliensis sp. nov., a new species isolated from the human gut and its taxonogenomic description

Bilen, Melhem, Fonkou, Maxime Descartes Mbogning, Dubourg, Grégory, Tomei, Enora, Richez, Magali, Delerce, Jérémy, Levasseur, Anthony, Daoud, Ziad, Raoult, Didier, Cadoret, Frédéric
Antonie van Leeuwenhoek 2019 v.112 no.6 pp. 935-945
Dysgonomonas, catalase, digestive system, feces, genome, genomics, humans, intestinal microorganisms, males, new species, nucleotide sequences, phenotype, phylogeny, proteomics, ribosomal RNA, sequence homology
Culturomics has allowed the isolation of a significant number of new bacterial species from the human gut microbiota and proved to be a valuable complement to culture-independent techniques. Using this culture-based approach, a new bacterial species has been isolated from a stool sample of a 39-year-old healthy Pygmy male and described using the taxonogenomic strategy. Cells of strain Marseille-P4356ᵀ are Gram-stain negative cocci. The strain grows optimally at 37 °C and is catalase positive but oxidase negative. Its 16S rRNA gene sequence exhibited 92.96% sequence similarity with Dysgonomonas gadei strain JCM 16698T (NR_113134.1), currently its phylogenetically closest species that has been validly named. The genome of strain Marseille-P4356ᵀ is 3,472,011 bp long with 37.3 mol% G+C content. Phenotypic, biochemical, proteomic, genomic and phylogenetic analyses, clearly demonstrate that strain Marseille-P4356ᵀ (= CCUG 71356ᵀ = CSUR P4356ᵀ) represents a new species within the genus Dysgonomonas, for which we propose the name Dysgonomonas massiliensis sp. nov.