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Dysgonomonas massiliensis sp. nov., a new species isolated from the human gut and its taxonogenomic description

Author:
Bilen, Melhem, Fonkou, Maxime Descartes Mbogning, Dubourg, Grégory, Tomei, Enora, Richez, Magali, Delerce, Jérémy, Levasseur, Anthony, Daoud, Ziad, Raoult, Didier, Cadoret, Frédéric
Source:
Antonie van Leeuwenhoek 2019 v.112 no.6 pp. 935-945
ISSN:
0003-6072
Subject:
Dysgonomonas, catalase, digestive system, feces, genome, genomics, humans, intestinal microorganisms, males, new species, nucleotide sequences, phenotype, phylogeny, proteomics, ribosomal RNA, sequence homology
Abstract:
Culturomics has allowed the isolation of a significant number of new bacterial species from the human gut microbiota and proved to be a valuable complement to culture-independent techniques. Using this culture-based approach, a new bacterial species has been isolated from a stool sample of a 39-year-old healthy Pygmy male and described using the taxonogenomic strategy. Cells of strain Marseille-P4356ᵀ are Gram-stain negative cocci. The strain grows optimally at 37 °C and is catalase positive but oxidase negative. Its 16S rRNA gene sequence exhibited 92.96% sequence similarity with Dysgonomonas gadei strain JCM 16698T (NR_113134.1), currently its phylogenetically closest species that has been validly named. The genome of strain Marseille-P4356ᵀ is 3,472,011 bp long with 37.3 mol% G+C content. Phenotypic, biochemical, proteomic, genomic and phylogenetic analyses, clearly demonstrate that strain Marseille-P4356ᵀ (= CCUG 71356ᵀ = CSUR P4356ᵀ) represents a new species within the genus Dysgonomonas, for which we propose the name Dysgonomonas massiliensis sp. nov.
Agid:
6431514