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Light-controlled thrombin catalysis and clot formation using a photoswitchable G-quadruplex DNA aptamer

Author:
Ali, Aysha, Bullen, Gemma A., Cross, Benjamin, Dafforn, Timothy R., Little, Haydn A., Manchester, Jack, Peacock, Anna F. A., Tucker, James H. R.
Source:
Chemical communications 2019 v.55 no.39 pp. 5627-5630
ISSN:
1364-548X
Subject:
anthracenes, blood coagulation, catalytic activity, dimerization, oligonucleotides, photochemical reactions, thrombin
Abstract:
The reversible photocontrol of an enzyme governing blood coagulation is demonstrated. The thrombin binding aptamer (TBA), was rendered photochromic by modification with two anthracene groups. Light-triggered anthracene photodimerisation distorts its structure, inhibiting binding of the enzyme thrombin, which in turn triggers catalysis and the resulting clotting process.
Agid:
6435547