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Host plant defenses of black (Solanum nigrum L.) and red nightshade (Solanum villosum Mill.) against specialist Solanaceae herbivore Leptinotarsa decemlineata (Say)

Author:
Ben‐Abdallah, Saoussen, Cáceres, Luis A., Wang, Zhiling, Renaud, B. Justin, Lachâal, Mokhtar, Karray‐Bouraoui, Najoua, Hannoufa, Abdelali, Scott, Ian M.
Source:
Archives of insect biochemistry and physiology 2019 v.101 no.2 pp. e21550
ISSN:
0739-4462
Subject:
Leptinotarsa decemlineata, Solanum nigrum, Solanum villosum, acetates, antifeeding activity, bioassays, enzyme inhibition, fat body, glutathione transferase, glycoalkaloids, herbivores, host plants, insecticidal properties, insecticide resistance, instars, larvae, leaf extracts, leaves, medicinal plants, methanol, midgut, mortality, secondary metabolites, toxicity, volatile compounds
Abstract:
Black nightshade (Solanum nigrum, S. nigrum L.) and red nightshade (Solanum villosum, S. villosum Mill.) are medicinal plants from the Solanaceae family that synthesize glycoalkaloids and other secondary metabolites. To recognize the potential insecticide activity of these compounds, leaf extracts (containing glycoalkaloid and methanol fractions) were tested for enzyme inhibition, antifeedant activity and toxicity. For in‐vitro glutathione S‐transferase (GST) inhibition activity, we used insecticide‐resistant Colorado potato beetle, Leptinotarsa decemlineata (L. decemlineata; Say) midgut and fat‐body homogenate. In‐vivo toxicity and the antifeedant activity were performed using larval bioassays. The methanol extracts had greater GST inhibitory activity compared to the glycoalkaloids, as well as greater 2nd instar larvae mortality and antifeedant activity. Furthermore, the green leaf volatile compound, cis‐hex‐3‐enyl acetate, at the concentration of 5 ppm, caused 50% mortality of 2nd instar larvae. Our findings suggest the potential usefulness of S. nigrum and S. villosum extracts to control L. decemlineata.
Agid:
6438057