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Nutritional recovery from a low-protein diet during pregnancy does not restore the kinetics of insulin secretion and Ca²⁺ or alterations in the cAMP/PKA and PLC/PKC pathways in islets from adult rats

Author:
Marin, Leonardo, Silva, Hellen Barbosa Faria, Damin, Gabriela, Ignacio-Souza, Letícia Martins, Reis, Sílvia Regina de Lima, de Oliveira, Camila Aparecida Machado, Ribeiro, Rosane Aparecida, Reis, Marise Auxiliadora de Barros, Latorraca, Márcia Queiroz, Ferreira, Fabiano, Arantes, Vanessa Cristina
Source:
Applied physiology, nutrition and metabolism 2018 v.43 no.12 pp. 1257-1267
ISSN:
1715-5320
Subject:
adults, cAMP-dependent protein kinase, calcium, cyclic AMP, glucose, insulin, insulin secretion, low protein diet, phospholipase C, pregnancy, protein content, protein kinase C, rats, rearing, suckling, weaning
Abstract:
We investigated the insulin release induced by glucose, the Ca²⁺ oscillatory pattern, and the cyclic AMP (cAMP)/protein kinase A (PKA) and phospholipase C (PLC)/protein kinase C (PKC) pathways in islets from adult rats that were reared under diets with 17% protein (C) or 6% protein (LP) during gestation, suckling, and after weaning and in rats receiving diets with 6% protein during gestation and 17% protein after birth (R). First-phase glucose-induced insulin secretion was reduced in LP and R islets, and the second phase was partially restored in the R group. Glucose stimulation did not modify intracellular Ca²⁺ concentration, but it reduced the Ca²⁺ oscillatory frequency in the R group compared with the C group. Intracellular cAMP concentration was higher and PKA-Cα expression was lower in the R and LP groups compared with the C group. The PKCα content in islets from R rats was lower than that in C and LP rats. Thus, nutritional recovery from a low-protein diet during fetal life did not repair the kinetics of insulin release, impaired Ca²⁺ handling, and altered the cAMP/PKA and PLC/PKC pathways.
Agid:
6439179