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Nutritional recovery from a low-protein diet during pregnancy does not restore the kinetics of insulin secretion and Ca²⁺ or alterations in the cAMP/PKA and PLC/PKC pathways in islets from adult rats

Marin, Leonardo, Silva, Hellen Barbosa Faria, Damin, Gabriela, Ignacio-Souza, Letícia Martins, Reis, Sílvia Regina de Lima, de Oliveira, Camila Aparecida Machado, Ribeiro, Rosane Aparecida, Reis, Marise Auxiliadora de Barros, Latorraca, Márcia Queiroz, Ferreira, Fabiano, Arantes, Vanessa Cristina
Applied physiology, nutrition and metabolism 2018 v.43 no.12 pp. 1257-1267
adults, cAMP-dependent protein kinase, calcium, cyclic AMP, glucose, insulin, insulin secretion, low protein diet, phospholipase C, pregnancy, protein content, protein kinase C, rats, rearing, suckling, weaning
We investigated the insulin release induced by glucose, the Ca²⁺ oscillatory pattern, and the cyclic AMP (cAMP)/protein kinase A (PKA) and phospholipase C (PLC)/protein kinase C (PKC) pathways in islets from adult rats that were reared under diets with 17% protein (C) or 6% protein (LP) during gestation, suckling, and after weaning and in rats receiving diets with 6% protein during gestation and 17% protein after birth (R). First-phase glucose-induced insulin secretion was reduced in LP and R islets, and the second phase was partially restored in the R group. Glucose stimulation did not modify intracellular Ca²⁺ concentration, but it reduced the Ca²⁺ oscillatory frequency in the R group compared with the C group. Intracellular cAMP concentration was higher and PKA-Cα expression was lower in the R and LP groups compared with the C group. The PKCα content in islets from R rats was lower than that in C and LP rats. Thus, nutritional recovery from a low-protein diet during fetal life did not repair the kinetics of insulin release, impaired Ca²⁺ handling, and altered the cAMP/PKA and PLC/PKC pathways.