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Isolation and characterization of a bacterium able to degrade high concentrations of iprodione

Cao, Li, Shi, Wenhong, Shu, Rundong, Pang, Jian, Liu, Yuetao, Zhang, Xiaohua, Lei, Yuming
Canadian journal of microbiology 2018 v.64 no.1 pp. 49-56
Microbacterium, bacteria, biodegradation, bioremediation, carbon, energy, gas chromatography-mass spectrometry, iprodione, mineralization, nucleotide sequences, phylogeny, ribosomal RNA
A bacterial strain CQH-1 capable of mineralizing iprodione was isolated and characterized. On the basis of its morphological, physiological, and biochemical characteristics combined with phylogenetic analysis of its 16S rRNA gene sequence, strain CQH-1 was identified as a Microbacterium sp. CQH-1. It was able to use iprodione and 3,5-dichloroaniline as the sole source of carbon and energy for its growth. It completely degraded 100 mg·L⁻¹ iprodione within 96 h at 30 °C. During the degradation of iprodione by strain CQH-1, 2 compounds were detected in GC–MS analysis and were recognized as N-(3,5-dichlorophenyl)-2,4-dioxoimidazolidine and 3,5-dichloroaniline. So, the biodegradation pathway of iprodione by strain CQH-1 was proposed. This is the first report of an iprodione-mineralizing strain from the genus Microbacterium, and strain CQH-1 might be a promising candidate for application in the bioremediation of iprodione-contaminated environments.