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H-NS represses transcription of the flagellin gene lafA of lateral flagella in Vibrio parahaemolyticus

Wang, Yan, Zhang, Yiquan, Yin, Zhe, Wang, Jie, Zhu, Yongzhe, Peng, Haoran, Zhou, Dongsheng, Qi, Zhongtian, Yang, Wenhui
Canadian journal of microbiology 2018 v.64 no.1 pp. 69-74
DNA-binding proteins, Vibrio cholerae, Vibrio parahaemolyticus, bacteria, bacterial motility, environmental factors, flagellin, flagellum, genes, transcription (genetics)
Swarming motility is ultimately mediated by the proton-powered lateral flagellar (laf) system in Vibrio parahaemolyticus. Expression of laf genes is tightly regulated by a number of environmental conditions and regulatory factors. The nucleoid-associated DNA-binding protein H-NS is a small and abundant protein that is widely distributed in bacteria, and H-NS-like protein-dependent expression of laf genes has been identified in Vibrio cholerae and V. parahaemolyticus. The data presented here show that H-NS acts as a repressor of the swarming motility in V. parahaemolyticus. A single σ²⁸-dependent promoter was detected for lafA encoding the flagellin of the lateral flagella, and its activity was directly repressed by H-NS. Thus, H-NS represses swarming motility by directly acting on lafA. Briefly, this work revealed a novel function for H-NS as a repressor of the expression of lafA and swarming motility in V. parahaemolyticus.