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H-NS represses transcription of the flagellin gene lafA of lateral flagella in Vibrio parahaemolyticus

Author:
Wang, Yan, Zhang, Yiquan, Yin, Zhe, Wang, Jie, Zhu, Yongzhe, Peng, Haoran, Zhou, Dongsheng, Qi, Zhongtian, Yang, Wenhui
Source:
Canadian journal of microbiology 2018 v.64 no.1 pp. 69-74
ISSN:
1480-3275
Subject:
DNA-binding proteins, Vibrio cholerae, Vibrio parahaemolyticus, bacteria, bacterial motility, environmental factors, flagellin, flagellum, genes, transcription (genetics)
Abstract:
Swarming motility is ultimately mediated by the proton-powered lateral flagellar (laf) system in Vibrio parahaemolyticus. Expression of laf genes is tightly regulated by a number of environmental conditions and regulatory factors. The nucleoid-associated DNA-binding protein H-NS is a small and abundant protein that is widely distributed in bacteria, and H-NS-like protein-dependent expression of laf genes has been identified in Vibrio cholerae and V. parahaemolyticus. The data presented here show that H-NS acts as a repressor of the swarming motility in V. parahaemolyticus. A single σ²⁸-dependent promoter was detected for lafA encoding the flagellin of the lateral flagella, and its activity was directly repressed by H-NS. Thus, H-NS represses swarming motility by directly acting on lafA. Briefly, this work revealed a novel function for H-NS as a repressor of the expression of lafA and swarming motility in V. parahaemolyticus.
Agid:
6439997