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Complete genome sequence of Enterobacter cloacae R11 reveals multiple genes potentially associated with high-level polymyxin E resistance

Author:
Zhong, Chuanqing, Zhang, Chao, Fu, Jiafang, Chen, Wenbing, Jiang, Tianyi, Cao, Guangxiang
Source:
Canadian journal of microbiology 2018 v.64 no.1 pp. 87-90
ISSN:
1480-3275
Subject:
Enterobacter cloacae, Escherichia coli, Gram-negative bacteria, antibiotic resistance genes, chromosomes, feedlots, multiple drug resistance, municipal wastewater, nucleotide sequences, plasmids, polymyxins, sequence analysis, swine, China
Abstract:
Enterobacter cloacae strain R11 is a multidrug-resistant bacterium isolated from sewage water near a swine feedlot in China. Strain R11 can survive in medium containing up to 192 μg/mL polymyxin E, indicating a tolerance for this antibiotic that is significantly higher than that reported for other gram-negative bacteria. In this study, conjugation experiments showed that partial polymyxin E resistance could be transferred from strain R11 to Escherichia coli strain 25922, revealing that some genes related to polymyxin E resistance are plasmid-based. The complete genome sequence of this strain was determined, yielding a total of 4 993 008 bp (G+C content, 53.15%) and 4908 genes for the circular chromosome and 4 circular plasmids. Genome analysis revealed a total of 73 putative antibiotic resistance genes, including several polymyxin E resistance genes and genes potentially involved in multidrug resistance. These data provide insights into the genetic basis of the polymyxin E resistance and multidrug resistance of E. cloacae.
Agid:
6440011