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Layer-by-layer assembled magnetic bimetallic metal-organic framework composite for global phosphopeptide enrichment
- Xiao, Ruili, Pan, Yini, Li, Jie, Zhang, Lingyi, Zhang, Weibing
- Journal of chromatography 2019 v.1601 pp. 45-52
- binding capacity, brain, chromatography, coordination compounds, magnetism, metal ions, moieties, phosphopeptides, proteomics, rats, skim milk, surface area
- The highly selective enrichment of phosphopeptides is vital for the research of phosphorylated proteomics. Herein, a magnetic bimetallic metal-organic framework (MOF) with Zr-O and Ti-O clusters (denoted as Fe3O4@SiO2@(Zr-Ti-PTA)15) has been fabricated using a layer-by-layer assembly approach via the coordination of metal ions with carboxyl groups. The composite has large specific surface area (135.84 m2 g−1) and superparamagnetism (62.03 emu g−1). Compared with single-metal cluster MOF, the Fe3O4@SiO2@(Zr-Ti-PTA)15 showed higher binding capacity (100 mg g−1), enhanced affinity, better selectivity (nβ-casein : nBSA = 1 : 2000) and sensitivity (0.3 fmol/μL) towards phosphopeptides due to the combination of the dual metal clusters. Furthermore, the Fe3O4@SiO2@ (Zr-Ti-PTA)15 was successfully applied to extracting phosphopeptides from non-fat milk tryptic digest, and 10 mono-phosphopeptides and 22 multi-phosphopeptides were captured. As a result, 1294 phosphopeptides (476 multi-phosphopeptides and 818 mono-phosphopeptides) were selectively extracted and detected from tryptic digest of rat brain extract, indicating its great application potential in phosphorylated proteomics research.