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Development of a loop-mediated isothermal amplification assay for Vibrio cholerae O1 and O139
- Izumiya, Hidemasa, Morita, Masatomo, Arakawa, Eiji, Ngo, Tuan Cuong, Nguyen, Hoai Thu, Nguyen, Dong Tu, Ohnishi, Makoto
- Molecular and cellular probes 2019 v.45 pp. 65-67
- Vibrio cholerae O1, bacteria, cholera, genes, loop-mediated isothermal amplification, rapid methods
- A loop-mediated isothermal amplification assay was developed. It was designed for recognizing Vibrio cholerae O1/O139, where atpA, rfbN, and wfbR genes were adopted. The assay specifically detected the target with sensitivities of 5–67 copies per reaction in 1 h. The assay will aid rapid detection of the cholera bacterium.