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Renaissance of traditional DNA transfer strategies for improvement of industrial lactic acid bacteria

Author:
Bron, Peter A, Marcelli, Barbara, Mulder, Joyce, van der Els, Simon, Morawska, Luiza P, Kuipers, Oscar P, Kok, Jan, Kleerebezem, Michiel
Source:
Current opinion in biotechnology 2019 v.56 pp. 61-68
ISSN:
0958-1669
Subject:
CRISPR-Cas systems, DNA, bacteriophages, gene editing, genomics, horizontal gene transfer, lactic acid bacteria, nanotubes
Abstract:
The ever-expanding genomic insight in natural diversity of lactic acid bacteria (LAB) has revived the industrial interest in traditional and natural genetic mobilization methodologies. Here, we review recent advances in horizontal gene transfer processes in LAB, including natural competence, conjugation, and phage transduction. In addition, we envision the possibilities for industrial strain improvement arising from the recent discoveries of molecular exchanges between bacteria through nanotubes and extracellular vesicles, as well as the constantly expanding genome editing possibilities using the CRISPR-Cas technology.
Agid:
6442782