Jump to Main Content
Optimization of enzymatic hydrolysis for effective lipid extraction from microalgae Scenedesmus sp.
- Zhang, Yi, Kong, Xiaoying, Wang, Zhongming, Sun, Yongming, Zhu, Shunni, Li, Lianhua, Lv, Pengmei
- Renewable energy 2018 v.125 pp. 1049-1057
- Fourier transform infrared spectroscopy, Scenedesmus, X-ray photoelectron spectroscopy, cell walls, cellulose, endo-1,4-beta-glucanase, enzymatic hydrolysis, enzymatic treatment, fatty acid methyl esters, hemicellulose, high performance liquid chromatography, lipids, microalgae, pH, polygalacturonase, renewable energy sources, response surface methodology, scanning electron microscopy, temperature, xylanases
- Cell wall disruption is an essential downstream processing step for improving the efficiency of lipid extraction from microalgae. Enzyme-assisted extraction of lipid from microalga Scenedesmus sp. with cellulase, xylanase and pectinase, using various parameters, such as enzyme concentration, temperature, pH and incubation time, was optimized by central composite design (CCD) coupled with response surface methodology (RSM). Both the lipid extraction from microalgae and the fatty acid methyl esters (FAMEs) production under optimal conditions showed a ∼twofold in the yields compared to the control group with no enzymatic treatment. SEM images, FTIR measurement, XPS and HPLC analysis showed that the enzymatic pretreatment caused significant alterations in the cell wall structure of microalgae. And the disruption of microalgal cell walls was primarily attributed to the breakage of β-glucosidic linkages in cellulose and hemicellulose. The study showed a promising approach can lead to an improvement in the lipid extraction yield from microalgae and further provide valuable information for the use of enzymes in microalgal processes.