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Exploring the potency of integrating semi-batch operation into lipid yield performance of Chlamydomonas sp. Tai-03

Tan, Chung Hong, Show, Pau-Loke, Ling, Tau Chuan, Nagarajan, Dillirani, Lee, Duu-Jong, Chen, Wei-Hsin, Chang, Jo-Shu
Bioresource technology 2019 v.285 pp. 121331
Chlamydomonas, biodiesel, biomass, carbon dioxide, diesel fuel, fatty acid methyl esters, fossil fuels, lipid content, microalgae, oleic acid, palmitic acid
Third generation biofuels, also known as microalgal biofuels, are promising alternatives to fossil fuels. One attractive option is microalgal biodiesel as a replacement for diesel fuel. Chlamydomonas sp. Tai-03 was previously optimized for maximal lipid production for biodiesel generation, achieving biomass growth and productivity of 3.48 ± 0.04 g/L and 0.43 ± 0.01 g/L/d, with lipid content and productivity of 28.6 ± 1.41% and 124.1 ± 7.57 mg/L/d. In this study, further optimization using 5% CO2 concentration and semi-batch operation with 25% medium replacement ratio, enhanced the biomass growth and productivity to 4.15 ± 0.12 g/L and 1.23 ± 0.02 g/L/d, with lipid content and productivity of 19.4 ± 2.0% and 239.6 ± 24.8 mg/L/d. The major fatty acid methyl esters (FAMEs) were palmitic acid (C16:0), oleic acid (C18:1), and linoleic acid (C18:2). These short-chain FAMEs combined with high growth make Chlamydomonas sp. Tai-03 a suitable candidate for biodiesel synthesis.