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Massive volumetric imaging of cleared tissue: The necessary tools to be successful

Author:
Watson, Alan M., Watkins, Simon C.
Source:
The international journal of biochemistry & cell biology 2019 v.112 pp. 76-78
ISSN:
1357-2725
Subject:
cellular microenvironment, image analysis, microscopes, optics, planning, technology
Abstract:
Recent technological innovations in high-speed microscopes, tissue clearing methods, optics and computation have made it possible to image enormous biologic volumes. These techniques are applicable to a broad range of biomedical disciplines and are becoming a necessary tool for exploring biologic systems that extend beyond cellular microenvironments to entire organs and organisms. Here we broadly discuss the tool sets that are available for cleared tissue imaging and how various choices may influence the planning and feasibility of an experiment.
Agid:
6443304