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Molecular investigation of tick-borne infections in cattle from Xinjiang Uygur Autonomous Region, China

Li, Yongchang, Li, Jixu, Chahan, Bayin, Guo, Qingyong, Zhang, Yang, Moumouni, Paul Franck Adjou, Lee, Seung-Hun, Liu, Mingming, Galon, Eloiza May, Guo, Huanping, Gao, Yang, Song, Ruiqi, Li, Min, Tumwebaze, Maria Agnes, Benedicto, Byamukama, Xuan, Xuenan
Parasitology international 2019
Anaplasma bovis, Babesia bigemina, Babesia bovis, Coxiella burnetii, cattle, cattle diseases, disease control, genes, pathogens, phylogeny, polymerase chain reaction, ribosomal DNA, ribosomal RNA, sequence analysis, subtropics, tick-borne diseases, China
Tick-borne diseases cause significant losses to livestock production in tropical and subtropical regions. However, information about the tick-borne infections in cattle in Xinjiang Uygur Autonomous Region (XUAR), northwestern China, is scarce. In this study, nested polymerase chain reaction (PCR) assays and gene sequencing were used to detect and analyze epidemiological features of Babesia bovis, B. bigemina, Coxiella burnetii and Anaplasma bovis infections in XUAR. Out of 195 samples tested, 24 (12.3%), 67 (34.4%), 40 (20.5%) and 10 (5.1%) were positive for B. bovis, B. bigemina, C. burnetii and A. bovis, respectively. Sequencing analysis indicated that B. bovis SBP-4, B. bigemina Rap1a, C. burnetii htpB and A. bovis 16S rRNA genes from XUAR showed 99%–100% identity with documented isolates from other countries. Phylogenetic analyses revealed that B. bovis SBP-4, B. bigemina Rap1a, C. burnetii htpB and A. bovis 16S rRNA gene sequences clustered in the same clade with isolates from other countries. To the best of our knowledge, this is the first report of C. burnetii infection of cattle in XUAR. Furthermore, this study provides important data for understanding the distribution of tick-borne pathogens, and is expected to improve the approach for prevention and control of tick-borne diseases in China.