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Autophagy and apoptosis in starved and refed Neocaridina davidi (Crustacea, Malacostraca) midgut

Włodarczyk, A., Student, S., Rost-Roszkowska, M.
Canadian journal of zoology 2019 v.97 no.4 pp. 294-303
B-lymphocytes, Malacostraca, adults, apoptosis, autophagy, epithelium, hepatopancreas, immunohistochemistry, intestines, midgut, refeeding, shrimp, starvation, transmission electron microscopy
Adult specimens of the freshwater shrimp Neocaridina davidi Bouvier, 1904 (Crustacea) were starved for 7, 14, and 21 days. Specimens from the first and second experimental group were collected for the studies. The majority of animals starved for 21 days died. Additionally, some specimens from each group were refed for 4, 7, and 14 days. The epithelium of the midgut, which is composed of the intestine and hepatopancreas, was analyzed. While the epithelium of the intestine is formed by D- and R-cells, the epithelium of the hepatopancreas has R-, B-, and F-cells. Autophagy and apoptosis in the midgut epithelium were analyzed using transmission electron microscopy and immunohistochemical methods. These processes were only observed in the D-cells of the intestine and the F- and B-cells of the hepatopancreas. Starvation led to a reduction in the amount of reserve material in the B-cells. Although this process activated autophagy in both regions of the midgut, the intestine and hepatopancreas, after refeeding, the level of autophagy decreased. Starvation caused an increase in the apoptotic cells in both organs, while the refeeding caused a decrease in the number of apoptotic cells in both organs analyzed. Refeeding after periods of starvation caused an accumulation of reserve material in the hepatopancreas.