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Considerations for incorporating real-time PCR assays into routine marine biosecurity surveillance programmes: a case study targeting the Mediterranean fanworm (Sabella spallanzanii) and club tunicate (Styela clava)

Author:
Wood, Susanna A., Pochon, Xavier, Ming, Witold, von Ammon, Ulla, Woods, Chris, Carter, Megan, Smith, Matt, Inglis, Graeme, Zaiko, Anastasija
Source:
Genome 2019 v.62 no.3 pp. 137-146
ISSN:
1480-3321
Subject:
DNA, RNA, Styela clava, biosecurity, case studies, invasive species, models, monitoring, quantitative polymerase chain reaction, risk, surveys, New Zealand
Abstract:
Molecular techniques may provide effective tools to enhance marine biosecurity surveillance. Prior to routine implementation, evidence-based consideration of their benefits and limitations is needed. In this study, we assessed the efficiency and practicality of visual diver surveys and real-time PCR assays (targeting DNA and RNA) for detecting two marine invasive species whose infestation levels varied between species and location: Sabella spallanzanii and Styela clava. Filtered water samples (n = 171) were collected in parallel with dive surveys at two locations as part of the New Zealand Marine High Risk Site Surveillance programme: Nelson Harbour (27 sites) and Waitemata Harbour (30 sites). Diver surveys resulted in a greater number of detections compared to real-time PCR: S. clava – 21 versus 5 sites in Nelson, 6 versus 1 in Auckland; S. spallanzanii – 18 versus 10 in Auckland, no detections in Nelson. Occupancy modelling derived detection probabilities for the real-time PCR for S. clava were low (14%), compared to S. spallanzanii (66%). This could be related to abundances, or species-specific differences in DNA shedding. Only one RNA sample was positive, suggesting that most detections were from extracellular DNA or non-viable fragments. While molecular methods cannot yet replace visual observations, this study shows they provide useful complementary information.
Agid:
6445540