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Enhanced endoxylanase production by Myceliophthora thermophila with applicability in saccharification of agricultural substrates

Dahiya, Seema, Singh, Bijender
3 Biotech 2019 v.9 no.6 pp. 214
Myceliophthora thermophila, ammonium nitrate, ammonium sulfate, calcium chloride, corn cobs, dipotassium hydrogen phosphate, endo-1,4-beta-xylanase, ferrous sulfate, inoculum, magnesium sulfate, pH, polysorbates, reducing sugars, rice straw, saccharification, secretion, solid state fermentation, spores, sucrose, surfactants, water activity, xylan, xylose
The production of enzymes by solid-state fermentation is an interesting process and currently used worldwide as it can be carried out in solid matrix in absence of free water. In present study, Myceliophthora thermophila BJTLRMDU3 produced high titres of endoxylanase (890.55 U/g DR, dry residue) using 5 g rice straw at pH 7.0 and at 45 °C with 1:7 (w/v) solid-to-moisture ratio with inoculum rate of 12 × 10⁶ spores/ml after 4 days in solid-state fermentation. High enzyme titre was produced after moistening the rice straw with solution containing ammonium sulphate (0.4%), K₂HPO₄ (1.0%), MgSO₄·7H₂O (0.3%), FeSO₄·7H₂O (0.03%) and CaCl₂ (0.03%). Addition of sucrose (2% w/v) and ammonium nitrate (2% w/v) further enhanced the endoxylanase production. A high endoxylanase production was achieved at water activity (aW) of 0.95 (1639.80 U/g DR) that declined drastically below this value. Among different surfactants, Tween 20 (3% v/v) enhanced the secretion of endoxylanase (2047.91 U/g DR). Furthermore, on optimization of K₂HPO₄ concentration, it was found that 0.5% K₂HPO₄ improved (2191.28 U/g DR) endoxylanase production and overall 4.35-folds increase in production of endoxylanase was achieved after optimization of culture conditions. The enzyme has potential to liberate monomeric (xylose) as well as oligomeric (xylotiose, xylotetrose, and xylopantose) sugars from xylan. On saccharification of rice straw and corncob with endoxylanase, maximum yield of reducing sugars was 135.61 and 132.61 mg/g of substrate recorded after 48, and 36 h, respectively.