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The Role of Neuropilin-1–FYN Interaction in Odontoblast Differentiation of Dental Pulp Stem Cells

Song, Yihua, Cao, Peipei, Gu, Zhifeng, Xiao, Jingwen, Lian, Min, Huang, Dan, Xing, Jing, Zhang, Ye, Feng, Xingmei, Wang, Chenfei
Cellular reprogramming 2018 v.20 no.2 pp. 117-126
cell differentiation, dental caries, drugs, enzymes, inflammation, ligands, signal transduction, stem cells, tooth pulp, transcription factor NF-kappa B
Abnormal odontoblast differentiation of dental pulp stem cells (DPSCs) caused by inflammation is closely related to the development of dental caries. Neuropilin-1 (NRP1) is one of the members of neuropilin family. It can combine with disparate ligands involved in regulating cell differentiation. FYN belongs to the protein-tyrosine kinase family, which has been implicated in the control of cell growth, and the effect can be further strengthened by inflammatory factors. In our studies, we verified that NRP1 can form complexes with FYN and have the correlation changes in odontoblast differentiation of DPSCs. Therefore, we surmise that in the progress of dental caries, NRP1 interacts with FYN, by expanding inflammation and inhibition of odontoblast differentiation of DPSCs through nuclear factor kappa B (NF-κB) signaling pathway. In this subject, we first investigated the expression and interaction of NRP1 and FYN in DPSCs. And then, we researched the effect of this complex controlling downstream signal pathway in normal or inflammation stimulated DPSCs. Finally, we analyzed the relationship between this role and odontoblast differentiation of DPSCs. This research will provide the molecular mechanism of inflammation factors of dental caries through activating NF-κB signal regulating odontoblast differentiation in DPSCs for finding new potential drug targets for the clinical treatment of dental caries.