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Effect of different concentrations of l-carnitine in extender for semen cryopreservation in sheep

Souza, Clara Vieira de, Brandão, Felipe Zandonadi, Santos, Juliana Dantas Rodrigues, Alfradique, Vivian Angélico Pereira, Santos, Vanessa Moreira Barbosa dos, Morais, Maria Clara da Cruz, Rangel, Paulo Sergio Cerqueira, Silva, Andreza Amaral da, Souza-Fabjan, Joanna Maria Gonçalves
Cryobiology 2019 v.89 pp. 104-108
carnitine, cryopreservation, egg yolk, freeze-thaw cycles, lipid peroxidation, oxidative stress, plasma membrane, rams, semen, semen extenders, spermatozoa
This study assessed the effect of l-carnitine (LC) in sheep semen extenders containing or not egg yolk for cryopreservation in sheep. Two extenders (TRIS-egg yolk or the commercial optiXcell™ IMV medium) were used, totaling six groups: IMV – (0, 5 and 10 mM LC) and TRIS – (0, 5 and 10 mM LC). After the freezing-thawing process and throughout incubation at 38 °C for up to 3 h, several parameters were evaluated: sperm kinetics, hypoosmotic, plasma membrane integrity, capacitation status and lipid peroxidation level. The supplementation of either 5 or 10 mM LC randomly affected some parameters and, overall, TRIS was superior (P < 0.05) than IMV extender. In the LC-groups, IMV had greater (P < 0.05) oxidative stress than TRIS. In conclusion, although LC affected isolated parameters, its supplementation in semen extender had no consistently beneficial effect on freezing-thawing ram sperm.