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Mitochondrial proteome profiling of Leishmania tropica

Author:
Tasbihi, Minoo, Shekari, Faezeh, Hajjaran, Homa, Masoori, Leila, Hadighi, Ramtin
Source:
Microbial pathogenesis 2019 v.133 pp. 103542
ISSN:
0882-4010
Subject:
Leishmania tropica, amino acids, electron transport chain, lipid metabolism, liquid chromatography, mitochondria, protein folding, proteins, proteome, tandem mass spectrometry, therapeutics, tricarboxylic acid cycle
Abstract:
The mitochondrion of kinetoplastida has unique characteristics both in structure and function. To better understand the mitochondrial proteome of the Leishmania tropica promastigote stage, liquid chromatography coupled with mass spectrometry (LC/MS/MS) approach was used. In the wake of mitochondria isolation and purity validation, 1212 proteins were identified, among which approximately 44% of proteins belonged to the mitochondrial proteome. Several functions were enriched in mitochondrial proteome including tricarboxylic acid cycle and respiratory chain, protein folding, signalling, transport, lipid metabolism, amino acid, and nucleotide metabolism. Furthermore, the result of the present research was compared with the previous related studies. Gaining more information about vital metabolism of the cell and molecules can be used for therapeutic purposes.
Agid:
6449959