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Complement proteins regulating macrophage polarisation on biomaterials

Araújo-Gomes, N., Romero-Gavilán, F., Zhang, Y., Martinez-Ramos, C., Elortza, F., Azkargorta, M., Martín de Llano, J.J., Gurruchaga, M., Goñi, I., van den Beucken, J.J.J.P., Suay, J.
Colloids and surfaces 2019 v.181 pp. 125-133
C-reactive protein, adsorption, biocompatibility, biocompatible materials, coatings, complement, inflammation, interleukin-10, macrophages, protein deposition, rabbits, secretion, tibia, titanium, tumor necrosis factor-alpha
One of the events occurring when a biomaterial is implanted in an host is the protein deposition onto its surface, which might regulate cell responses. When a biomaterial displays a compromised biocompatibility, distinct complement pathways can be activated to produce a foreign body reaction. In this article, we have designed different types of biomaterial surfaces to study the inflammation process. Here, we used different concentrations of (3-glycidoxypropyl)-trimethoxysilane (GPTMS), an organically-modified alkoxysilane as a precursor for the synthesis of various types of sol-gel materials functionalizing coatings for titanium implants to regulate biological responses. Our results showed that greater GPTMS surface concentrations induced greater secretion of TNF-α and IL-10 on RAW 264.7 macrophages. When implanted into rabbit tibia, osseointegration decreased with higher GPTMS concentrations. Interestingly, higher deposition of complement-related proteins C-reactive protein (CRP) and ficolin-2 (FCN2), two main activators of distinct complement pathways, was observed. Taking all together, inflammatory potential increase seems to be GPTMS concentration-dependent. Our results show that a greater adsorption of complement proteins can condition macrophage polarization.