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Avoidance behaviour of isopods (Porcellio scaber) exposed to food or soil contaminated with Ag- and CeO2- nanoparticles

Author:
Zidar, Primož, Kos, Monika, Ilič, Eva, Marolt, Gregor, Drobne, Damjana, Jemec Kokalj, Anita
Source:
Applied soil ecology 2019 v.141 pp. 69-78
ISSN:
0929-1393
Subject:
Collembola, Porcellio scaber, adverse effects, avoidance behavior, ceric oxide, earthworms, food choices, food contamination, leaves, metal ions, nanoparticles, nanosilver, pollutants, polluted soils, silver, toxicity
Abstract:
The main goal of the study was to evaluate the avoidance behaviour of isopods (Porcellio scaber) to food or soil contaminated with different nanoparticles (NPs): Ag-NPs, sulfidized Ag-NPs (Ag2S-NPs) or CeO2-NPs. These NPs are characterized by different physiochemical properties, and antimicrobial and toxic potentials. Isopods were exposed in three scenarios: (i) 14 d feeding test where animals were fed solely on contaminated leaves; (ii) 14 d food selection test where leaves spiked with NPs were offered simultaneously with uncontaminated leaves; and (iii) 48 h soil avoidance test where animals could choose between uncontaminated and soil spiked with NPs. The isopods decreased feeding on Ag-NP contaminated food and clearly avoided contact with Ag-NP contaminated soil. Substantial accumulation of Ag in the body of isopods was found both in feeding and food selection test. This means that food selection might mitigate but could not prevent Ag accumulation in woodlice. In contrast, no such behavioural response of isopods was found for CeO2-NP. These data suggest that the presence of metal ions in the case of Ag-NPs may be the main reason for differential behaviour. Interestingly, moderate avoidance behaviour was observed also towards Ag2S-NPs although no Ag+ was detected in stock suspensions. This avoidance was not statistically significant and needs to be further explored. Behavioural test with isopods showed again their value in estimation of potential adverse effects of pollutants comparable to collembolans and earthworms.
Agid:
6450087