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Molecular characterization of methanogenic microbial communities for degrading various types of polycyclic aromatic hydrocarbon

Ye, Quanhui, Liang, Chengyue, Chen, Xunwen, Fang, Tingting, Wang, Yun, Wang, Hui
Journal of environmental sciences (China) 2019 v.86 pp. 97-106
Acinetobacter, Arcobacter, Methanobacterium, Thauera, Thiobacillus, anthracenes, bacteria, bioremediation, carboxylation, community structure, methanogens, microbial communities, naphthalene, phenanthrenes, ribosomal RNA, sequence analysis
Knowledge on methanogenic microbial communities associated with the degradation of polycyclic aromatic hydrocarbons (PAHs) is crucial to developing strategies for PAHs bioremediation. In this study, the linkage between the type of PAHs and microbial community structure was fully investigated through 16S rRNA gene sequencing on four PAH-degrading cultures. Putative degradation products were also detected. Our results indicated that naphthalene (Nap)/2-methylnaphthalene (2-Nap), phenanthrene (Phe) and anthracene (Ant) sculpted different microbial communities. Among them, Nap and 2-Nap selected for similar degrading bacteria (i.e., Alicycliphilus and Thauera) and methanogens (Methanomethylovorans and Methanobacterium). Nap and 2-Nap were probably activated via carboxylation, producing 2-naphthoic acid. In contrast, Phe and Ant shaped different bacterial and archaeal communities, with Arcobacter and Acinetobacter being Phe-degraders and Thiobacillus Ant-degrader. Methanogenic archaea Methanobacterium and Methanomethylovorans predominated Phe-degrading and Ant-degrading culture, respectively. These findings can improve our understanding of natural PAHs attenuation and provide some guidance for PAHs bioremediation in methanogenic environment.