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Static hydrothermal processing and fractionation for production of a collagen peptide with anti-oxidative and anti-aging properties

Park, Sung Hee, Jo, Yeon-Ji
Process biochemistry 2019 v.83 pp. 176-182
anti-aging properties, antioxidant activity, antioxidants, bioavailability, collagen, collagenase, dietary supplements, enzyme inhibition, fish skin, fractionation, hot water treatment, humans, hydrolysates, hydrolysis, industry, ingredients, molecular weight, peptides, synergism, temperature, ultrafiltration
Collagen, in a form suitable for human consumption, can be obtained from the large amounts of fish skin via protein hydrolysis to produce low-molecular-weight substances with enhanced bioavailability and function. To optimize this method, we investigated the synergistic effect of elevated temperature (150–250 °C) and pressure (350–3900 kPa) of a hydrothermal process on the hydrolytic ability and characteristics of collagen hydrolysates. Elevated temperature and pressure of the hydrothermal process increased the levels of free amino groups and lower-molecular-weight collagen hydrolysates, particularly at 210 °C and 2100 kPa. The resulting hydrolysates were fractionated by ultrafiltration membranes of different molecular weight cutoff and evaluated for their antioxidant (ABTS radical scavenging activity and reducing power) and anti-aging (tyrosinase and collagenase inhibition) activities. The <1 kDa fraction had the highest antioxidant activities, whereas the 5–10 kDa fraction had the highest anti-aging activities. Therefore, fish skin could be successfully modified into biologically active collagen peptides by a hydrothermal process (hydrolysis) and ultrafiltration (separation), and the resulting bioactive peptides have potential for development as antioxidants and anti-aging ingredients in the food, nutraceutical, and cosmetic industries.