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Static hydrothermal processing and fractionation for production of a collagen peptide with anti-oxidative and anti-aging properties
- Park, Sung Hee, Jo, Yeon-Ji
- Process biochemistry 2019 v.83 pp. 176-182
- anti-aging properties, antioxidant activity, antioxidants, bioavailability, collagen, collagenase, dietary supplements, enzyme inhibition, fish skin, fractionation, hot water treatment, humans, hydrolysates, hydrolysis, industry, ingredients, molecular weight, peptides, synergism, temperature, ultrafiltration
- Collagen, in a form suitable for human consumption, can be obtained from the large amounts of fish skin via protein hydrolysis to produce low-molecular-weight substances with enhanced bioavailability and function. To optimize this method, we investigated the synergistic effect of elevated temperature (150–250 °C) and pressure (350–3900 kPa) of a hydrothermal process on the hydrolytic ability and characteristics of collagen hydrolysates. Elevated temperature and pressure of the hydrothermal process increased the levels of free amino groups and lower-molecular-weight collagen hydrolysates, particularly at 210 °C and 2100 kPa. The resulting hydrolysates were fractionated by ultrafiltration membranes of different molecular weight cutoff and evaluated for their antioxidant (ABTS radical scavenging activity and reducing power) and anti-aging (tyrosinase and collagenase inhibition) activities. The <1 kDa fraction had the highest antioxidant activities, whereas the 5–10 kDa fraction had the highest anti-aging activities. Therefore, fish skin could be successfully modified into biologically active collagen peptides by a hydrothermal process (hydrolysis) and ultrafiltration (separation), and the resulting bioactive peptides have potential for development as antioxidants and anti-aging ingredients in the food, nutraceutical, and cosmetic industries.