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Developmental expression patterns of fosl genes in Xenopus tropicalis

Guo, Xiao-Fang, Zhang, Zhou, Zheng, Li, Zhou, Yi-Min, Wu, Hai-Yan, Liang, Chi-Qian, Zhao, Hui, Cai, Dong-Qing, Qi, Xu-Feng
Gene expression patterns 2019 v.34 pp. 119056
Xenopus tropicalis, antigens, cell cycle, embryogenesis, eyes, genes, in situ hybridization, liver, lungs, quantitative polymerase chain reaction
Fos-like antigens (Fosl) including Fosl1 and Fosl2 exclusively heterodimerize with Jun members to form AP-1 complex, thereby participating in various cellular progresses including cell cycle regulation. However, expression patterns of these two genes during embryonic development remains largely unknown. In the present study, both temporal and spatial expression patterns of fosl1 and fosl2 were examined during embryonic development of Xenopus tropicalis. Real-time quantitative PCR results showed that the expression of the two genes was increased from stage 2 to stage 42. However, expression level of fosl1 is much higher than that of fosl2 at stage 42. Whole-mount in situ hybridization showed that fosl1 was expressed in eyes, branchial arch, notochord, otic vesicle, and liver. However, fosl2 was expressed in lung primordium from stage 34 to stage 38, in addition to the moderate expression in eyes and branchial arch at stage 42. Thus, the developmental expression patterns of these two fosl genes is different in Xenopus embryos. These results provide a basis for further functional study of these two genes.