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Autophagy induced by avian reovirus enhances viral replication in chickens at the early stage of infection

Author:
Niu, Xiaosai, Zhang, Chengcheng, Wang, Yuyang, Guo, Mengjiao, Ruan, Baoyang, Wang, Xuefeng, Wu, Tianqi, Zhang, Xiaorong, Wu, Yantao
Source:
BMC veterinary research 2019 v.15 no.1 pp. 173
ISSN:
1746-6148
Subject:
Avian orthoreovirus, autophagy, chickens, in vitro studies, interleukin-1beta, pathogens, poultry diseases, tissues, virus replication
Abstract:
BACKGROUND: Avian reovirus (ARV) is an important pathogen that can cause serious disease in poultry. Though several in vitro studies revealed some molecular mechanisms that are responsible for ARV-induced autophagy, it is still largely unknown how ARV manipulates autophagy to promote its own propagation. RESULTS: In this study, we demonstrated that ARV infection triggered autophagy in chicken tissues, evident from the enhancement of LC3-I/−II conversion and the appearance of abundant autophagosomes. Moreover, viral replication and the expression of IL-1β were coupled with the process of ARV-induced autophagy in the early stage of infection. Furthermore, regulation of autophagy affected the accumulation of LC3-II, the production of ARV and the expression of IL-1β. CONCLUSIONS: Altogether, our data suggest that ARV induces autophagy, which benefits its replication and dissemination in chicken tissues at the early infection stage.
Agid:
6453070