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Extraction of steviol glycosides from dried Stevia rebaudiana by pressurized hot water extraction

Németh, Á., Jánosi, Sz.
Acta alimentaria 2019 v.48 no.2 pp. 241-252
Stevia rebaudiana, color, glycosides, leaf extracts, leaves, pressing, response surface methodology, solvents, spray drying, steviol, stevioside
In existing processes, the extraction of steviol glycosides from stevia leaves involves many process steps often including extraction by organic solvents. The purpose of the present study was to develop a process for the effective extraction of steviol glycosides, which can provide a concentrated juice exhibiting a high level of recovery with regard to the target compounds, rebaudioside A and stevioside. Pressurized Hot Water Extraction (PHWE) was first optimized with Response Surface Methodology in terms of maximized rebaudioside-A yield and minimized colour components. PHWE was then combined with pressing in a wine-press, resulting higher efficiency for extracting both steviol glycosides in comparison to the reported methods in the literature. Finally, spray drying was applied for both product stabilization and removal of contaminants.