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Transport and retention of Shewanella oneidensis strain MR1 in water-saturated porous media with different grain-surface properties

Ning, Zigong, Li, Rong, Lian, Keting, Liao, Peng, Liao, Hehuan, Liu, Chongxuan
Chemosphere 2019 v.233 pp. 57-66
Shewanella oneidensis, bacteria, calcium, cations, dolomite, electrostatic interactions, energy, ionic strength, kinetics, magnesium, porous media, quartz, sodium
Bacterium Shewanella oneidensis strain (MR1), a facultative microorganism that plays critical role in contaminant transformation and degradation, was used as an example to decipher the transport and retention of microorganisms in water-saturated porous media with different grain-surface properties and under different ionic compositions (i.e. Na+, Ca2+, and Mg2+). Dolomite and quartz sands, which contained different surface charge properties, were used as the representative minerals. Dolomite was selected because its surface charges are significantly affected by solution composition. The mobility of MR1 in the dolomite column was lower than that in the quartz column, because the lower energy barrier between MR1 and dolomite than that between MR1 and quartz, resulting in the larger retention of MR1 in the dolomite column. The breakthrough curves were well simulated by the two sites kinetic model with HYDRUS-1D. The maximum concentration of attached bacteria (Smax) were positively correlated to the ionic strength regardless of mineral types. The values of Smax were about 1.1–4.0 times larger in the MR1-dolomite system than that in the MR1-quartz system under different ionic strength conditions. The retention of the MR1 on dolomite surfaces in the presence of divalent cations Ca2+ is significantly higher than that on quartz surfaces primarily due to the larger electrostatic attraction energy between the MR1 and dolomite grains. The findings demonstrate that the porous media with the lower negative charge has the higher capacity for the retention and deposition of MR1, potentially affecting the transport of MR1 and other bacteria in the subsurface.