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Redox and global interconnected proteome changes in mice exposed to complex environmental hazards surrounding Doñana National Park

Author:
Michán, Carmen, Chicano-Gálvez, Eduardo, Fuentes-Almagro, Carlos A., Alhama, José
Source:
Environmental pollution 2019 v.252 pp. 427-439
ISSN:
0269-7491
Subject:
Mus, adenosine triphosphate, amino acid metabolism, antioxidants, biotransformation, blood coagulation, cholesterol metabolism, environmental hazards, enzymes, fatty liver, habitats, homeostasis, inflammation, mice, mining, models, national parks, oxidation, oxidative stress, pollutants, pollution, protein synthesis, proteins, proteolysis, proteome, proteomics, toxic substances, Spain
Abstract:
Natural environments are receiving an increasing number of contaminants. Therefore, the evaluation and identification of early responses to pollution in these complex habitats is an urgent and challenging task. Doñana National Park (DNP, SW Spain) has been widely used as a model area for environmental studies because, despite its strictly protected core, it is surrounded by numerous threat sources from agricultural, mining and industrial activities. Since many pollutants often induce oxidative stress, redox proteomics was used to detect redox-based variations within the proteome of Mus spretus mice captured in DNP and the surrounding areas. Functional analysis showed that most differentially oxidized proteins are involved in the maintenance of homeostasis, by eliciting mechanisms to respond to toxic substances and oxidative stress, such as antioxidant and biotransformation processes, immune and inflammatory responses, and blood coagulation. Furthermore, changes in the overall protein abundance were also analysed by label-free quantitative proteomics. The upregulation of phase I and II biotransformation enzymes in mice from Lucio del Palacio may be an alert for organic pollution in the area located at the heart of DNP. Metabolic processes involved in protein turnover (proteolysis, amino acid catabolism, new protein biosynthesis and folding) were activated in response to oxidative damage to these biomolecules. Consequently, aerobic respiratory metabolism increased to address the greater ATP demands. Alterations of cholesterol metabolism that could cause hepatic steatosis were also detected. The proteomic detection of globally altered metabolic and physiological processes offers a complete view of the main biological changes caused by environmental pollution in complex habitats.
Agid:
6455126