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Application of the kinetic and isotherm models for better understanding of the mechanism of biomineralization process induced by Purpureocillium lilacinum Y3

Xia, Mingchen, Bao, Peng, Liu, Ajuan, Li, Shen, Yu, Runlan, Liu, Yuandong, Li, Jiaokun, Wu, Xueling, Huang, Caoming, Chen, Miao, Qiu, Guanzhou, Zeng, Weimin
Colloids and surfaces 2019 v.181 pp. 207-214
Purpureocillium lilacinum, X-ray diffraction, biomineralization, biosynthesis, fluorescence, jarosite, metal ions, models, mycelium, polymers, transmission electron microscopy
Purpureocillium lilacinum can promote the biomineralization of jarosite by secreting extracellular polymeric substances (EPS), but the detailed mechanism is not clear. In this study, the biosynthesis process of jarosite induced by P. lilacinum Y3 and hypha cell surface characterization were investigated. X-ray diffraction (XRD) analysis indicated that P. lilacinum Y3 could induce the formation of jarosite crystal and enhance mineralization kinetics. The kinetic and isotherm models confirmed that the metal ions transferring from the solution to the mycelium surface was controlled by diffusion process and the active interfacial sites on hypha cell surface played a pivotal role in the biomineralization process. Furthermore, transmission electron microscopy (TEM) pictures illustrated that the P. lilacinum Y3 mainly induced the generation of mineral precipitate extracellularly, but not intracellularly. Three-dimensional excitation-emission matrix (3D-EEM) fluorescence spectrum results further revealed the extracellular compounds such as fulvic-acid-like and protein-like substances participated in the mineralization process.