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Viability of Lactobacillus plantarum encapsulated with poly-γ-glutamic acid produced by Bacillus sp. SJ-10 during freeze-drying and in an in vitro gastrointestinal model

Author:
Jang, Won Je, Choi, Sun-Young, Lee, Jong Min, Lee, Ga Hye, Hasan, Md Tawheed, Kong, In-Soo
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2019 v.112 pp. 108222
ISSN:
0023-6438
Subject:
Bacillus (bacteria), Lactobacillus plantarum, adhesion, bacteria, bile salts, encapsulation, freeze drying, gastric juice, human cell lines, intestines, models, pH, polyglutamic acid, probiotics, survival rate, viability
Abstract:
In the present study, the probiotic Lactobacillus plantarum was encapsulated with 0.1%, 0.25%, or 0.5% 400-kDa poly-γ-glutamic acid (γ-PGA400) produced by Bacillus sp. SJ-10. The viability of the encapsulated cells was assessed under various stress conditions that are common to the processing and ingestion of probiotics, such as freeze-drying, exposure to simulated gastric juice (SGJ), and exposure to bile salt. During freeze-drying to make powder, L. plantarum levels decreased by 1.50 log colony forming units (CFU)/ml without encapsulation. When encapsulated with 0.5% γ-PGA400 under the same conditions, L. plantarum levels decreased by 0.19 log CFU/ml. In the SGJ condition (pH 2), all L. plantarum bacteria died within 1 h without encapsulation but exhibited the highest viability (decrease of 0.30 log CFU/ml) when encapsulated with 0.5% γ-PGA400. All groups had a high survival rate in the bile salt condition (pH 5.9). In the intestinal adhesion test with Caco-2 cells, the highest rate of adherence was 35.9% when the cells were encapsulated with 0.25% γ-PGA400. The present findings suggest that γ-PGA400 as an encapsulating material increases the viability of L. plantarum under various stress conditions.
Agid:
6457334