PubAg

Main content area

Structural properties and antioxidant activities of polysaccharide from fruit bodies of Pholiota nameko

Author:
Zhu, Zhen-Yuan, Pan, Li-Chao, Han, Dan, Sun, Hui-qing, Chen, Li-Jing
Source:
Natural product research 2019 v.33 no.11 pp. 1563-1569
ISSN:
1478-6427
Subject:
Fourier transform infrared spectroscopy, Pholiota microspora, antioxidant activity, antioxidants, fruiting bodies, glucose, mannose, molecular weight, nuclear magnetic resonance spectroscopy, polyphenols, polysaccharides, spectrophotometers, sugar content, ultraviolet-visible spectroscopy
Abstract:
A polysaccharide named PNP was extracted and purified from Pholiota nameko. The total sugar content of PNP was 95.29% and the molecular weight was 1.89 × 10³ kDa. The structural features of PNP were investigated by the combination of chemical and instrumental analysis such as UV spectrophotometer, specific rotation determination, FT-IR, methylisation analysis and Congo red. The results showed that the optical rotation of PNP was +120° and that it had a triple-helical structure. Besides, PNP was mainly composed of glucose and mannose at the molar ratio of 4.24:1.00. The backbone of PNP was composed of (1→3)-linked-Glc and (1→3)-linked-Man whereas the branches of (1→3,6)-linked- Glc, (1→3,6)-linked-Man and T- Glc. Consistenting with the results of UV–Vis spectra, FT-IR spectroscopy and ¹H NMR, indicated that PNP was a complex of polysaccharides and polyphenols. In vitro antioxidant results suggested that PNP was processed with certain scavenging capacity.
Agid:
6458316