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Antioxidant and anti-proliferative properties of extracts from heterotrophic cultures of Galdieria sulphuraria

Bottone, Claudia, Camerlingo, Rosa, Miceli, Roberta, Salbitani, Giovanna, Sessa, Giuseppe, Pirozzi, Giuseppe, Carfagna, Simona
Natural product research 2019 v.33 no.11 pp. 1659-1663
adenocarcinoma, antioxidant activity, apoptosis, cell cycle checkpoints, cytotoxicity, glutathione, human health, humans, microalgae, phycocyanin, propidium, protective effect, therapeutics
This study explores the possibility to use the extremophilic microalga Galdieria sulphuraria (strain 064) as a source of natural biomolecules with beneficial and protective effects on human health. Galdieria was cultivated in heterotrophy conditions and cells extracts for their antioxidant and anti-proliferative properties were tested. Galdieria extracts showed high antioxidant power tested through ABTS assay and revealed high glutathione and phycocyanin contents. Based on Annexin-V FITC/propidium iodide and MTT analysis, algae extracts inhibited the proliferation of human adenocarcinoma A549 cells (51.2% inhibition) through the induction of apoptosis without cell cycle arrest. Besides, cytotoxicity and cytometry assays showed a positive pro-apoptotic mechanism. On these bases, we suggest that G. sulphuraria from heterotrophic culture, for its therapeutic potential, could be considered a good candidate for further studies with the aim to isolate bioactive anti-cancer molecules.