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Antioxidant and anti-proliferative properties of extracts from heterotrophic cultures of Galdieria sulphuraria
- Bottone, Claudia, Camerlingo, Rosa, Miceli, Roberta, Salbitani, Giovanna, Sessa, Giuseppe, Pirozzi, Giuseppe, Carfagna, Simona
- Natural product research 2019 v.33 no.11 pp. 1659-1663
- adenocarcinoma, antioxidant activity, apoptosis, cell cycle checkpoints, cytotoxicity, glutathione, human health, humans, microalgae, phycocyanin, propidium, protective effect, therapeutics
- This study explores the possibility to use the extremophilic microalga Galdieria sulphuraria (strain 064) as a source of natural biomolecules with beneficial and protective effects on human health. Galdieria was cultivated in heterotrophy conditions and cells extracts for their antioxidant and anti-proliferative properties were tested. Galdieria extracts showed high antioxidant power tested through ABTS assay and revealed high glutathione and phycocyanin contents. Based on Annexin-V FITC/propidium iodide and MTT analysis, algae extracts inhibited the proliferation of human adenocarcinoma A549 cells (51.2% inhibition) through the induction of apoptosis without cell cycle arrest. Besides, cytotoxicity and cytometry assays showed a positive pro-apoptotic mechanism. On these bases, we suggest that G. sulphuraria from heterotrophic culture, for its therapeutic potential, could be considered a good candidate for further studies with the aim to isolate bioactive anti-cancer molecules.