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Proteomic profiling of Sporotrichum thermophile under the effect of ionic liquids: manifestation of an oxidative stress response
- Sadaf, Ayesha, Sinha, Rajeshwari, Khare, Sunil K.
- 3 Biotech 2019 v.9 no.6 pp. 240
- H+/K+-exchanging ATPase, Myceliophthora thermophila, catalase, fungi, glycolysis, in vitro studies, ionic liquids, oxidative stress, peroxidase, protein synthesis, proteins, proteomics, stress response, tricarboxylic acid cycle
- Sporotrichum thermophile, a known producer of industrial enzymes exhibited stability in the presence of ionic liquids (ILs).The study reports, for the first time, the stress response of S. thermophile upon exposure to ILs. In vitro assay showed increased anti-oxidative enzyme levels indicating ROS-mediated oxidative stress by ILs. The proteomic profile and identification of differential proteins confirmed the fungal adaptations by (i) increased expression of glycolytic enzymes and ATP synthases (ii) downregulation of TCA cycle and protein synthesis machinery components (iii) expression of HSP70 and catalase/peroxidase. These changes are indicative of metabolic regulation of many important pathways and how ILs can be used to manipulate protein behavior.