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A novel fluorescent assay for the ultrasensitive detection of miRNA-21 with the use of G-quadruplex structures as an immobilization material for a signal indicator
- Yang, Ze-Zhou, Wen, Zhi-Bin, Peng, Xin, Chai, Ya-Qin, Liang, Wen-Bin, Yuan, Ruo
- Chemical communications 2019 v.55 no.45 pp. 6453-6456
- DNA, chemical reactions, fluorescence, gene amplification, protoporphyrin
- In this work, a novel fluorescent assay was proposed for the ultrasensitive detection of microRNA-21 (miRNA-21) based on the efficient immobilization of protoporphyrin IX (PPIX) as signal indicators in massive G-quadruplex structures obtained by target recycling, three-dimensional DNA walker and a rolling circle amplification (RCA) coupled cascade nucleic acid amplification strategy.